王昭雯教授

教授
王昭雯

English
電話:+886-6-275-7575 ext.58120    研究室：58104 轉29
email: cwwang02_ncku@gs.ncku.edu.tw
研究專長：分子細胞生物學、胞器生成及膜運輸。

王昭雯博士是一位細胞生物學家，專門研究胞器動力學和膜運輸。 她在 Daniel Klionsky 博士的實驗室攻讀博士學位，研究自噬作用。她加入了加州大學柏克萊分校 Randy Schekman 博士的實驗室進行博士後研究，利用胞外重組方法頗析將蛋白質從反式高爾基體網絡運送到細胞膜的分子機制。自 2007 年加入中央研究院植物暨微生物學研究所以來，王昭雯博士的研究主要集中在利用酵母菌和線蟲作為模式生物來揭示油滴生成與功能的分子機制。 她的團隊採用生物化學、分子生物學、細胞生物學和遺傳學等方法來探究細胞中油滴的生成與油滴降解的分子機制。王昭雯博士於 2023 年 8 月加入國立成功大學生命科學系，新團隊將在既定努力的基礎上，為推進油滴和膜動力學的研究貢獻新的視角。

研究方向：

油滴的生成機制和油脂恆定調節

油滴是一個細胞用來儲存中性脂肪和專司油脂代謝的胞器。油滴的細胞學研究在近年大幅地成長，在不同的細胞形式，油滴的大小和數目不盡相同，這也反映出不同細胞中油脂代謝的意義不同。油滴與細胞中幾乎所有的胞器進行互動，參與油脂的運送和代謝。近年來，隨著油滴在細胞中可能參與多種嶄新的功能也開始被廣泛地發表，當今細胞學上針對油滴的研究，已跳脫油滴在在油脂代謝層次的單一思維。

我們的研究目標是全方位地瞭解油滴生成及降解機制、及油滴在參與細胞各種複雜現象所扮演的角色及其背後的調控邏輯。我們利用酵母菌作為模式生物系統來幫助我們有效率的篩選突變株，並逐步釐清油滴相關蛋白的分子功能。我們建構胞外系統，以期更進一步分析油滴相關蛋白所參與的分子機制。

 

脂質與膜的細胞生物學

脂質是構成生物膜的基本成分。 每個細胞中的胞器都具有獨特的形狀和專司的功能，也擁有獨特的膜脂質與蛋白質的組合。我們的研究重點在於了解不同的膜脂質如何有助於建立胞器的特性、影響膜蛋白的結構和功能、以及如何參與細胞活動。為了解決這些問題，我們透過操縱脂質生成酵素來改變油脂的組成，並採用了結合遺傳學、生物化學、脂質組學和細胞生物學的多種方法來探究脂質如何參與細胞機制的調控。

學歷

博士 加州大學戴維斯分校 細胞暨發育生物學博士
碩士 臺灣大學植物病理學研究所
學士
 臺灣大學植物病理學系

經歷

年份 職位 機構
2023.8 – 迄今  教授 國立成功大學 生命科學系
2022.3 – 2023.7 研究員 中央研究院 植物暨微生物學研究所
2017.8 – 2022.3 長聘副研究員 中央研究院 植物暨微生物學研究所
2012.3 – 2017.8 副研究員 中央研究院 植物暨微生物學研究所
2007.5 – 2012.3 助研究員 中央研究院 植物暨微生物學研究所
2004.1 – 2007.4 博士後研究 加州大學柏克萊分校
2003.3 – 2003.12 博士後研究 密西根大學安那堡分校

榮譽及獎勵

年份 榮譽及獎勵
2014 中央研究院Career Development Award
2012 中央研究院年輕學者著作獎
2012 吳健雄基金會台灣傑出女科學家新秀獎

代表著作

  1. Cheng, Y.-L., Kuan, J.-E., Wang, C.-W., Chen, R.-H* (2025) Mga2-mediated transcription supports mitotic nuclear expansion under lipid saturation conditions in stearoyl-CoA desaturase Ole1 mutant. Biol. Cell; 36(11):ar144.
  2. Wang, C.-W.*, Chiu, P., Monsalve, S., Roure, R., Bai, X., Law, J.-J., Wu, Y.-C., Che, Y.-R., Cheng, Y.-L., Che, R.-H., Wu, Y.-C. (2025) High-glucose diets modulate phosphatidylcholine metabolism and fecundity in Caenorhabditis elegans. Cell Dev. Bio.,13:1622695. (*Corresponding author)
  3. Banerjee, S., Daetwyler, S., Bai, X., Michaud, M., Jouhet, J., Madhugiri, S., Johnson, E., Wang, C.-W., Fiolka, R., Toulmay, A., Prinz, W.-A.* (2025) The Vps13-like protein BLTP2 is pro-survival and regulates phosphatidylethanolamine levels in the plasma membrane to maintain its fluidity and function. Cell Biol., 27(7):1125-1135.
  4. Han, H.-F., Nien, S.-F., Jiang, H.-S., Wu, J.-C., Chiang, C.-Y., Li, M.-T., Huang, L.-J., Chiang, S., Lin, L.-C., Chuang, Y.-T., Lin, Y.-H., Wang, C.-W., Wu, Y.-C.* (2024). Dietary bacteria control elegans fat content through pathways converging at phosphatidylcholine. eLife, 96473.1.
  5. Wang, C.-W.*, Chen, R.-H., Chen, Y.-K. (2024) The lipid droplet assembly complex consists of seipin and four accessory factors in budding yeast. Biol Chem. 300(8):107534. (*Corresponding author)
  6. Li, Y.-H., Ke, T.-Y., Shih, W.-C., Liou, R-F.*, and Wang, C.-W.* (2021) NbSOBIR1 partitions into plasma membrane microdomains and binds ER-localized Nb Front Plant Sci. 12: 721548. (*Corresponding author)
  7. Bai, X., Huang, L.-J., Chen, S.-W., Nebenfuehr, B., Wysolmerski, B., Wu, J.-C., Olson, S. K., Golden, A., and Wang, C.-W.* (2020) Loss of the seipin gene perturbs eggshell formation in Caenorhabditis elegans. Development. 147(20):dev192997. (*Corresponding author)
  8. Su, W.-C., Lin, Y.-H., Pagac, M., and Wang, C.-W.* (2019) Seipin negatively regulates sphingolipid production at the ER-LD contact site. J Cell Biol. 218(11):3663-3680. (*Corresponding author)
  9. Hsu, T.-H., Chen, R-H., Cheng, Y.-H., and Wang, C.-W.* (2017) Lipid droplets are central organelles for meiosis II progression during yeast sporulation. Biol. Cell. 28(3):440-451. (*Corresponding author)
  10. Iwasa, S., Sato, N., Wang, C.-W., Cheng, Y.-H., Irokawa, H., Hwang. G.W., Naganuma, A., Kuge, S. (2016) The phospholipid:diacylglycerol acyltransferase Lro1 is responsible for Hepatitis C Virus core-induced lipid droplet formation in a yeast model system. PLoS One. 11(7):e0159324.
  11. Yang, P.-L,, Hsu, T.-H., Wang C.-W.*, Chen, R.-H.*. (2016) Lipid droplets maintain lipid homeostasis during anaphase for efficient cell separation in budding yeast. Mol Biol Cell. 27(15):2368-80. (*Corresponding author)
  12. Wang, C.-W.* (2016). Lipid droplets, lipophagy, and beyond. Biochim Biophys Acta. 1861 (8 Pt B):793-805. (Invited Review) (*Corresponding author)
  13. Wang, C.-W.* (2015). Lipid droplet dynamics in budding yeast. Cell Mol Life Sci. 72(14):2677-95. (Invited Review) (*Corresponding author)
  14. Peng, K.-C., Wang, C.-W., Wu, C.-H., Huang, C.-T., Liou, R.-F. (2015). Tomato SOBIR1/EVR homologs are involved in elicitin perception and plant defense against the oomycete pathogen Phytophthora parasitica. Mol Plant Microbe Interact. 28(8):913-26.
  15. Wang, C.-W.* (2014). Stationary phase lipophagy as a cellular mechanism to recycle sterols during quiescence. Autophagy. 10(11): 2075-6. (*Corresponding author)
  16. Wang, C.-W.*, Miao, Y.-H., and Chang, Y.-S. (2014). A sterol-enriched vacuolar microdomain mediates stationary phase lipophagy in budding yeast. J Cell Biol. 206(3):357-66. (*Corresponding author)

Selected for highlight in J. Cell Biol. 206 (3): 330. (2014)

Faculty of 1000 “recommended”

  1. Wang, C.-W.*, Miao, Y.-H., and Chang, Y.-S. (2014). Control of lipid droplet size in budding yeast requires the collaboration between Fld1 and Ldb16. J Cell Sci. 127: 1214-1228. (*Corresponding author)
  2. Starr, T. L., Pagant, S., Wang, C.-W., and Schekman, R. (2012). Sorting signals that mediate traffic of chitin synthase III between the TGN/endosomes and to the plasma membrane in yeast. PLoS One. 7(10): e46386.
  3. Wang, C.-W.*, and Lee, S.-C. (2012). The ubiquitin-like (UBX)-domain-containing protein Ubx2/Ubxd8 regulates lipid droplet homeostasis. J Cell Sci. 125: 2930-2939. (*Corresponding author)
  4. Wu, C.-H., Lee, S.-C., and Wang, C.-W.* (2011). Viral protein targeting to the cortical endoplasmic reticulum is required for cell-cell spreading in plants. J Cell Biol. 193 (3): 521-535. (*Corresponding author)

                Selected for highlight in J Cell Biol. 193 (3): 426. (2011)

                Selected for highlight in Nature Reviews Microbiology. 9: 400. (2011)

  1. Lee, S.-C., Wu, C.-H., and Wang, C.-W.* (2010). Traffic of a viral movement protein complex to the highly curved tubules of the cortical endoplasmic reticulum. 11, 912-930. (*Corresponding author)
  2. Wang, C. -W., Hamamoto, S., Orci, L., and Schekman, R. Exomer: A coat complex for transport of select membrane proteins from the trans-Golgi network to the plasma membrane in yeast. (2006). J Cell Biol. 174(7): 973-83.

 Selected for highlight in Nature Reviews Molecular Cell Biology, 7: 795. (2006).

  1. Cheong, H., Yorimitsu, T., Reggiori, F., Legakis, J. E., Wang, C. -W., and Klionsky, D. J. (2005) Atg17 regulates the magnitude of the autophagic response. Mol Biol Cell. 16(7): 3438-53.
  2. Stromhaug, P. E., Reggiori, F., Guan, J., Wang C.-W., and Klionsky DJ. (2004). Atg21 is a phosphoinositide binding protein required for efficient lipidation and localization of Atg8 during uptake of aminopeptidase I by selective autophagy. Mol Biol Cell. 15(8): 3553-3566.
  3. Reggiori, F., Wang, C.-W., Stromhaug, P. E., Abeliovich, H., and Klionsky, D. J. (2004). Early stages of the secretory pathway but not endosomes, are required for Cvt vesicle and autophagosome assembly in Saccharomyces cerevisiae. Biol. Cell. 15(5): 2189-204.
  4. Wang, C.-W., Stromhaug, P. E., Kauffman, E., Weisman, L. S., and Klionsky, D. J., (2003). Yeast homotypic vacuole fusion requires the Ccz1-Mon1 complex during the tethering/docking stage. J Cell Biol. 163(5): 973-85.
  5. Wang, C.-W., and Klionsky, D. J., (2003). The molecular mechanism of autophagy. Molecular Medicine. 9(3/4): 65-76.
  6. Wang, C.-W. and Klionsky, D. J. (2003). Chapter 8: Microautophagy. In: Autophagy. D. J. Klionsky ed. Landes Bioscience. Georgetown, TX.
  7. Reggiori, F., Wang, C.-W., Stromhaug, P. E., Shintani, T., and Klionsky, D. J. (2003). Vps51 is part of the yeast Vps Fifty Three tethering complex essential for retrograde traffic from the early endosome and Cvt vesicle completion. J Biol Chem. 278(7): 5009-5020.
  8. Wang, C.-W., Stromhaug, P. E., Shima, J. and Klionsky, D. J. (2002). The Ccz1-Mon1 protein complex is required for the late step of multiple vacuole delivery pathways. J Biol Chem. 277(49): 47917-27.
  9. Wang, C.-W., Kim, J., Huang, W-P., Abeliovich, H., Stromhaug, P. E., Dann, W. A. Jr., and Klionsky, D. J. (2001). Apg2 is a novel protein required for the cytoplasm to vacuole targeting, autophagy, and pexophagy pathways. J Biol Chem. 276(32): 30442-51.

 全部論文著作連結